eDNA as a Tool for Inferring Insect Species Presence and Characterizing Communities

Organizer(s)

• 

  Jeff Everett

  Fish and Wildlife Biologist
  Ecological Services, Oregon Fish & Wildlife Office
  U.S. Fish and Wildlife Service
  Portland, Oregon
• DP

  David S. Pilliod

  U.S. Geological Survey
  Boise, Idaho
• 

  Tabitha Graves (she/her/hers)

  Research Ecologist
  Northern Rocky Mountain Science Center
  US Geological Survey
  West Glacier, Montana

This state-of-the-science symposium will cover using eDNA techniques to understand insect communities. Experts will present lessons learned about sample designs and field methods to maximize results, DNA degradation, novel lab techniques, and how this can be applied to management and research questions for communities and species of concern.

Presentations:

• 8:00 AM - 8:05 AM Pacific

  Introductory remarks
  

  Presenting Author: David S. Pilliod – U.S. Geological Survey
• 8:05 AM - 8:20 AM Pacific

  Stability of pollinator environmental DNA (eDNA) on flowers under laboratory and field conditions
  

  Presenting Author: Muruleedhara Byappanahalli – U.S. Geological Survey

  Co-Author: Dawn Shively – USGS, Great Lakes Science Center

  Co-Author: Noel Pavlovic – USGS, Great Lakes Science Center

  Co-Author: Leslie Reyes – USGS, Great Lakes Science Center
• 8:20 AM - 8:35 AM Pacific

  Development and validation of an eDNA assay for detection of the endangered Franklin's bumble bee (Bombus franklini)
  

  Presenting Author: Michaela R. Grossklaus – U.S. Geological Survey

  Co-Author: David S. Pilliod – U.S. Geological Survey

  Co-Author: Matthew Laramie – U.S. Geological Survey, Forest and Rangeland Ecosystem Science Center

  Co-Author: Yer Lor – U.S. Geological Survey, Upper Midwest Environmental Science Center

  Co-Author: Marissa Kaminski – U.S. Geological Survey

  Co-Author: Stephen Spear – U.S. Geological Survey, Upper Midwest Environmental Science Center

  Co-Author: Jeff Everett – U.S. Fish and Wildlife Service
• 8:35 AM - 8:50 AM Pacific

  Detection of the threatened Dakota skipper (Hesperia dacotae) from flower-derived eDNA
  

  Presenting Author: David S. Pilliod – U.S. Geological Survey

  Co-Author: Michaela R. Grossklaus – U.S. Geological Survey

  Co-Author: Stacie Kageyama – U.S. Geological Survey, Upper Midwest Environmental Sciences Center

  Co-Author: Cale Nordmeyer – Minnesota Zoo

  Co-Author: Jerry Reinisch – U.S. Fish and Wildlife Service, North Dakota Ecological Services Field Office

  Co-Author: Erik Runquist – Minnesota Zoo
• 8:50 AM - 9:05 AM Pacific

  Targeted assay development and metabarcoding insights for detecting Bombus species on eDNA samples
  

  Presenting Author: Marissa Kaminski – U.S. Geological Survey

  Co-Author: David S. Pilliod – U.S. Geological Survey

  Co-Author: Jonathan Koch – USDA-ARS-PWA-PIRU

  Co-Author: Yer Lor – U.S. Geological Survey, Upper Midwest Environmental Science Center

  Co-Author: Matthew Laramie – U.S. Geological Survey, Forest and Rangeland Ecosystem Science Center

  Co-Author: Muruleedhara Byappanahalli – U.S. Geological Survey

  Co-Author: Ralph Grundel – Great Lakes Science Center

  Co-Author: Dawn Shively – USGS, Great Lakes Science Center

  Co-Author: Ashley Spoljaric – U.S. Geological Survey, Lake Michigan Ecological Research Station

  Co-Author: Amanda Boone – U.S. Geological Survey, Forest and Rangeland Ecosystem Science Center

  Co-Author: Kristina Parker – U.S. Geological Survey, Forest and Rangeland Ecosystem Science Center

  Co-Author: Michael Branstetter – USDA-ARS

  Co-Author: Aaron Aunins – U.S. Geological Survey, Leetown Science Center

  Co-Author: Kara Jones – U.S. Geological Survey, Eastern Ecological Science Center

  Co-Author: Stephen Spear – U.S. Geological Survey, Upper Midwest Environmental Science Center
• 9:05 AM - 9:20 AM Pacific

  Sampling needs for bee species of concern using qPCR of environmental DNA from flowers
  

  Presenting Author: Eliza Stein – Northern Rocky Mountain Science Center
• 9:20 AM - 9:35 AM Pacific

  Break and poster session
• 9:20 AM - 9:35 AM Pacific

  Integrating targeted surveys during community-wide occupancy assessments efficiently improves detection rates for species of concern
  

  Presenting Author: Tabitha Graves (she/her/hers) – US Geological Survey
• 9:35 AM - 9:50 AM Pacific

  Bee better: Leveraging multiple primer sets improves efficacy of eDNA metabarcoding to detect native bees
  

  Presenting Author: Sara Merkelz – University of Illinois

  Co-Author: Sara Merkelz – University of Illinois

  Co-Author: Amy Flansburg – Stantec

  Co-Author: Ashley Bennett – Electric Power Research Institute

  Co-Author: Mark Davos – University of Illinois Urbana-Champaign
• 9:50 AM - 10:05 AM Pacific

  Combining eDNA and vane traps to characterize pollinator communities and their associations with at-risk and invasive flora
  

  Presenting Author: Aron Katz – US Army
• 10:05 AM - 10:20 AM Pacific

  Testing the capacity of eDNA for sampling bee communities
  

  Presenting Author: Nevin Cullen – University of Oregon
• 10:20 AM - 10:35 AM Pacific

  Resurveying bees at Indiana Dunes National Park using traditional and eDNA methods
  

  Presenting Author: Ralph Grundel – Great Lakes Science Center
• 10:35 AM - 10:50 AM Pacific

  Leveraging eDNA and eRNA to better understand pathogen prevalence on floral resources
  

  Presenting Author: Mark Davis – University of Illinois

  Co-Author: Elena Gratton – University of Illinois

  Co-Author: Sara Merkelz – University of Illinois

  Co-Author: Elise Snyder – University of Illinois Urbana-Champaign

  Co-Author: Ashley Bennett – Electric Power Research Institute

  Co-Author: Amy Flansburg – Stantec
• 10:50 AM - 11:05 AM Pacific

  Break
• 11:05 AM - 11:20 AM Pacific

  Detecting habitat restoration effects on native pollinators – sampling needs for eDNA
  

  Presenting Author: Gavin Cotterill – Northern Rocky Mountain Science Center
• 11:20 AM - 11:35 AM Pacific

  The state of the science of flower-derived environmental DNA for pollinator biodiversity monitoring and conservation
  

  Presenting Author: Ashley Rohde – New Mexico State University
• 11:35 AM - 12:00 PM Pacific

  Panel discussion