RNA interference in Diaprepes abbreviatus: A tool for functional genomics and identification of potential pest management targets

Diaprepes abbreviatus is a major pest of citrus, responsible for extensive root damage and significant yield losses. Its complex life cycle complicates effective management, and the limited availability of genomic and transcriptomic resources has restricted molecular studies and hindered our understanding of its biology. RNA interference (RNAi) offers a powerful approach for functional genomics and holds promise as an environment-friendly pest control strategy. In this study, we investigated the effects of silencing multiple gene targets on the mortality and development of D. abbreviatus. Double-stranded RNA (dsRNA) was delivered to neonates and early-instar larvae through oral feeding, and we monitored their development through to adult emergence. Several gene targets significantly affected life history traits, resulting in increased mortality, developmental malformations, and altered life table parameters. These results demonstrate that RNAi can be an effective and selective method for controlling D. abbreviatus when gene-specific sequences are used to minimize off-target effects on non-target organisms.